Coding

Part:BBa_I719002:Design

Designed by: Lucas Hadjilucas   Group: iGEM07_Imperial   (2007-07-13)


Top Secret Vanilla Synthesiser


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 811
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 811
    Illegal NotI site found at 755
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 811
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 811
    Illegal NgoMIV site found at 184
    Illegal NgoMIV site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

TGA -> TAATAA


Source

Genbank accession number: AJ536325 REFERENCE 1 

AUTHORS   Barghini,P., Civolani,C., Ficca,A.G., Schiesser,A. and Ruzzi,M.

TITLE     Cloning and Characterization of Ferulate Catabolism Genes from

          Pseudomonas fluorescens BF13

JOURNAL   Unpublished

REFERENCE 2 (bases 1 to 831) 

AUTHORS   Ruzzi,M.

TITLE     Direct Submission

JOURNAL   Submitted (09-JAN-2003) Ruzzi M., Agrobiology and Agrochemistry,

          DABAC - University of Tuscia, via C. de Lellis, snc, Viterbo -

          01100, ITALY

References